Isolation of influenza C virus recombinants.

Isolation and Identification of Avian Influenza Virus H9N2 Subtype

An investigation of some factors affecting cross-reactivation between influenza A viruses.

Some parameters of the reactivation of ultravioletor chemically inactivated fowl plague virus by live A 2 viruses were examined. Clones of reactivated virus were obtained by two different techniques, either by isolation from plaques on chick embryo fibroblast cells or by terminal dilution in pieces of allantois-on-shell. Recombinants isolated from plaques were of one type, namely they contained...

In vitro and in vivo effects of Peganum harmala L. seeds extract against influenza A virus

Objective: Influenza A virus infections are still a major health problem and the choices available for the control and treatment of the disease are limited. This research evaluated in vitro and in vivo antiviral effects of Peganum harmala L. seeds (PHS) extract against influenza A virus. Materials and Methods: In this research, in vitro anti-influenza A virus activity of the extract was assesse...

Recombinant cold-adapted attenuated influenza A vaccines for use in children: molecular genetic analysis of the cold-adapted donor and recombinants.

A previously described cold-adapted attenuated virus, A/Leningrad/134/17/57 (H2N2), was further modified by 30 additional passages in chicken embryos at 25 degrees C. This virus had a distinct temperature-sensitive (ts) phenotype, grew well in chicken embryos at 25 degrees C, and failed to recombine with reference ts mutants of fowl plague virus containing ts lesions in five genes coding for no...

Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses

Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans. Highly pathogenic avian influenza viruses (HPAI) H5N1 were first identified in Southeast Asia in 1996 and spread to four continents over the following years. The viruses have caused high mortality in chickens and various bird species ...